FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40636835
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTT203000.018.2015271
TGCGGGT184350.018.0226971
GACCAAT164550.017.7127741
ATGCGAT124750.017.6607821
ATAGACC130350.017.6008031
GCGCGAT92650.017.3459111
TGACTAG132550.017.3086741
CTCGAAA123150.017.2903331
CCCTCCT207050.017.1514241
TGCCAAA145250.017.0145911
GGGAGAT117700.016.9989551
CGATGGC181250.016.9127121
CGTTCTG249750.016.8691391
TGCACCT174400.016.8168221
GGGTTGC169350.016.8163191
GAATGAA134400.016.8068731
ACGAGGA125550.016.7824761
CGCTGGA133300.016.7633551
CTCTACG117150.016.7591291
GAACTTG126350.016.7543542
Produced by FastQC (version 0.11.5)