FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699009
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT415800.3274271244315206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103350.050.931119
GGTATCA110050.047.788398
TGGTATC107100.047.7311337
AAGCAGT143000.040.171871
GTGGTAT137900.037.303736
AGCAGTG152050.036.8233572
CAGTGGT149650.035.2657624
AGTGGTA151300.034.4860425
ATCAACG102350.031.39511710-11
CAACGCA118300.028.01771412-13
AGAGTAC106250.027.40958418-19
TCAACGC110200.026.96926712-13
ACGCAGA121250.026.95727514-15
AACGCAG126900.024.00006514-15
GCAGTGG243200.023.0232583
TATCAAC123550.022.15704210-11
GCAGAGT134400.022.01088116-17
CGCAGAG134400.021.95957416-17
GTACTTT116700.021.20161422-23
CAGAGTA140800.019.230818-19
Produced by FastQC (version 0.11.5)