FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22382285
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA251630.11242373153589992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT72450.017.878251
CGGACTG68100.017.5844821
GACTAAC60500.017.3442551
GGGACCT61050.017.2540441
CTTTGCG70350.017.1510331
GGGAATG71400.017.1105791
GCGGGTT67950.017.089261
GTGATAA70000.017.0356228
ACTGATG56650.016.9749571
TGACTAG71850.016.9192371
CCACTAC73400.016.7130151
CTGATAG82300.016.6938421
TCTAACT85950.016.6654058
TGGCTGG87350.016.6634351
CGGACGT77450.016.6589951
CGTCTAC73650.016.6152251
CTCTACG67300.016.5803171
GGTATTG75450.016.566191
AGCCTAA88250.016.5506081
TGGCCAG72000.016.533991
Produced by FastQC (version 0.11.5)