FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22032792
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA247420.11229625369313158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT72100.018.0446591
GACTAAC62350.017.499281
CGGACTG67050.017.47691
CTTTGCG66400.017.2071651
GCGGGTT69500.017.194831
TGACTAG68750.017.1915551
GGGAATG69550.017.1244181
GTGATAA70800.017.0253968
AAACCTG64850.017.0114421
TGGCTGG82150.016.9920431
TGGCCAG72150.016.8990211
TGACGGC57000.016.8929121
CCCTCCT106250.016.8236641
GGGAGAT45600.016.822081
TGATTTC65400.016.8066461
ATGCGAT53900.016.7971131
GGGACCT59250.016.7113531
CCACTAC71550.016.6598551
TCTAACT84750.016.6052768
CTCTACG71850.016.6043421
Produced by FastQC (version 0.11.5)