FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45345695
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA510730.11263031694629447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT141300.017.5629231
CGGACTG137600.017.560381
GACTAAC127600.017.2666381
GCGGGTT143100.017.22261
CTTTGCG141450.017.1605831
GTGATAA139850.017.0834088
ATGCGAT116100.017.0723231
GGGACCT124050.016.9910241
CTGATAG165050.016.9661561
TGGCTGG172500.016.950111
GGGAATG142900.016.9301871
GGGCATC149900.016.8369351
TGACTAG143300.016.8057731
ACTGATG113050.016.76831
GGTATTG151550.016.6867831
CGTCTAC160250.016.6840521
CGGACGT154000.016.6497461
TCTAACT171400.016.644948
CCACCTA190450.016.576961
TGGCCAG144900.016.502281
Produced by FastQC (version 0.11.5)