FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40363054
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT151800.017.9966771
CCACCTA243550.017.8212951
ACTGATG138950.017.5490191
TACTTGT169050.017.4905661
GTAACTG165350.017.4001371
GAAACTC188450.017.381011
GATGCTA144650.017.3733941
GGGCATC151950.017.3417851
CCGGTAG163250.017.31511
ATGCGAT103250.017.2877431
CGGACTG125000.017.272761
TACTTAC201050.017.2347161
CGGAGTC185900.017.2288481
CTAAGAC131800.017.1314371
CCACTAC131750.017.1302851
TGGACGC127800.017.099491
TCTGGAA142800.017.0688231
TGAGCAT161500.017.0281581
TATGCCC118700.017.0255871
CGCTGGA111750.016.938381
Produced by FastQC (version 0.11.5)