FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24062958
Sequences flagged as poor quality0
Sequence length98
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69050.035.9269459
GTGGTAT90000.027.5134226
ATCAACG54350.027.30160910-11
GGTATCA92950.026.0462978
AGTGGTA118950.021.0875685
TCAACGC67250.020.7661812-13
ACGCAGA87800.017.9737514-15
AGAGTAC75900.017.88599618-19
CAGTGGT146800.017.3687274
TGGTATC141750.017.0793887
CAACGCA107050.014.8478912-13
CTACACG15850.013.5214931
AGCAGTG203100.013.0981322
GTACTTT90500.013.079185522-23
TATCAAC104550.012.56687310-11
AACGCAG124050.011.99926714-15
AAGCAGT237150.011.6304011
GCAGAGT132850.010.65089616-17
CGCAGAG157550.09.2435316-17
GCAGTGG334950.08.6554583
Produced by FastQC (version 0.11.5)