FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13210385
Sequences flagged as poor quality0
Sequence length98
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19500.022.52794610-11
GTATCAA28400.022.3518569
TCAACGC23800.017.87794512-13
GGTATCA40150.015.3522528
GTGGTAT39400.015.0603416
ACGCAGA35450.012.45726314-15
CTACACG7057.2759576E-1212.429341
AGTGGTA55000.011.7086455
AGAGTAC30650.011.03120218-19
CAACGCA46550.010.22759312-13
CAGTGGT68750.09.8352624
GTCGTAT4200.0085996898.7846461
TAGCGCG73550.08.75568292
TGGTATC67550.08.6481787
TATCAAC44800.08.16285210-11
AACGCAG55650.08.10081214-15
GTACTTT38150.07.89748622-23
GGGAAGT101400.07.802523692
AGCAGTG103350.07.7442632
GACGAAT22850.07.6697461
Produced by FastQC (version 0.11.5)