FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687082
Sequences flagged as poor quality0
Sequence length98
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT554470.4370350881313764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120100.050.398859
GGTATCA125550.048.5703358
TGGTATC125150.047.968747
AAGCAGT153050.042.5491871
AGCAGTG158900.040.5003172
GTGGTAT156000.038.91636
CAGTGGT158750.038.5560574
AGTGGTA164850.036.9638755
ATCAACG115050.031.10917710-11
CAACGCA131850.027.9161412-13
TCAACGC123250.026.77150212-13
ACGCAGA136150.026.7032714-15
AGAGTAC128000.026.26921518-19
GCAGTGG253100.025.8078943
AACGCAG140100.024.00250414-15
GCAGAGT153000.022.42099216-17
CGCAGAG149650.022.1542816-17
TATCAAC141900.021.91694810-11
GTACTTT131800.021.9116122-23
CTACACG10000.020.192721
Produced by FastQC (version 0.11.5)