FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17560364
Sequences flagged as poor quality0
Sequence length98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG10900.027.0770661
GTATCAA36600.023.1255239
ATCAACG33300.020.78964610-11
GGTATCA47650.017.6661958
ACGACGC17000.017.0464555
TCAACGC45750.014.27749712-13
TACACGA20650.014.03340152
ACGCAGA49400.013.96785314-15
CAACGCA56200.013.01414512-13
TGGTATC60450.012.7076837
AGAGTAC44750.010.79337518-19
ACGCTCT28400.010.3661268
CACGACG28200.010.2762324
AACGCAG68950.010.00742414-15
CGCAGAG75100.08.75905216-17
CAGTGGT99050.08.7306344
AGTGGTA96600.08.7139775
AGCAGTG125200.08.4501792
AAGCAGT135500.08.2021151
TCCGATC32500.07.6428063
Produced by FastQC (version 0.11.5)