FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17306435
Sequences flagged as poor quality0
Sequence length98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33750.025.0790029
CTACACG10750.020.6501391
ATCAACG33750.020.1028710-11
GGTATCA50500.017.2161738
TCAACGC47500.014.23487212-13
ACGACGC16300.014.1097235
ACGCAGA51200.014.01423514-15
TGGTATC60100.013.9303857
CAACGCA57900.013.14768412-13
TACACGA18050.012.9980432
AGAGTAC44750.010.84471418-19
AACGCAG73000.010.58525114-15
CAGTGGT95400.09.9806324
AGTGGTA94800.09.4615525
AGCAGTG128700.09.3292562
AAGCAGT133250.09.1627731
GTGGTAT101200.08.6819866
CACGACG28250.08.4668324
CGCAGAG78200.08.29377516-17
ACGCTCT28400.08.2607238
Produced by FastQC (version 0.11.5)