FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27194844
Sequences flagged as poor quality0
Sequence length98
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68000.030.3510069
ATCAACG60650.026.10913510-11
GTGGTAT87750.021.5293626
TCAACGC68150.021.21137812-13
GGTATCA97000.020.4240578
ACGCAGA99700.016.96631414-15
AGTGGTA123400.015.8685515
AGAGTAC73000.014.92343218-19
CAACGCA118800.014.25722912-13
CAGTGGT150050.013.1115914
TGGTATC149250.012.596377
AACGCAG139500.011.58206514-15
TATCAAC108800.011.17457110-11
AGCAGTG227800.010.3124522
AAGCAGT271600.09.84826851
GTACTTT89750.09.44915922-23
CTACACG15400.09.014931
TAGCGCG153900.08.81019592
GCAGAGT136900.08.57891316-17
CGCAGAG181700.08.34841216-17
Produced by FastQC (version 0.11.5)