FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28358781
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCTA168450.017.9903741
GGGACCT104400.017.9081751
GAAACTC129950.017.6657561
GGGCATC105050.017.5206261
GTAACTG118050.017.4679241
CCACTAC96050.017.450661
TACTTGT117750.017.3932381
ACTGATG96200.017.3400861
TCTGGAA99300.017.313621
TGGACGC93200.017.2958981
CGGACTG83200.017.2541161
ATGCGAT71900.017.2330681
CGCTGGA78750.017.2234521
CTAAGAC94000.017.1913591
CCGGTAG114800.017.176531
TATGCCC83150.017.1198161
GATGCTA102700.017.1113591
TGCGCAG136100.017.1105561
TACTTAC145950.017.0822351
CGGAGTC129250.017.0323581
Produced by FastQC (version 0.11.5)