FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28358781
Sequences flagged as poor quality0
Sequence length98
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72100.032.470899
ATCAACG64250.027.02422110-11
GGTATCA96900.023.1635258
TCAACGC71650.022.21090312-13
GTGGTAT94950.022.1865166
ACGCAGA104650.017.4931414-15
AGTGGTA128900.016.7712655
CAACGCA129650.014.84667712-13
AGAGTAC80150.014.60584118-19
CAGTGGT162250.014.0345124
TGGTATC157500.013.5210087
TATCAAC111450.012.03006210-11
AACGCAG152200.011.86175514-15
AGCAGTG239250.011.5015482
CTACACG16650.010.5403931
AAGCAGT281800.010.4068711
GTACTTT92700.010.0724722-23
TAGCGCG158450.08.8830792
GCAGAGT147850.08.78859716-17
CGCAGAG192750.08.38790116-17
Produced by FastQC (version 0.11.5)