FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44404455
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA502600.11318684127527294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT142650.017.8502241
CGGACTG135550.017.7043081
GGGACCT119650.017.3968011
CTTTGCG140700.017.3148211
GCGGGTT136400.017.2532141
GACTAAC125650.017.2094171
TGGCTGG162650.017.1773411
GGGAATG141250.017.1687091
CTGATAG158100.016.9750521
ACTGATG113400.016.9655821
ATGCGAT111950.016.9416261
GTGATAA140150.016.9017478
CTCGAGG121250.016.8172251
TGGCCAG142350.016.8088931
CGGACGT153000.016.7946051
CTCTACG139300.016.7924791
TCTAACT169650.016.778868
TGACTAG138800.016.721931
GGGAGAT91250.016.6544321
CGTCTAC149600.016.6156881
Produced by FastQC (version 0.11.5)