FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42960634
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA477030.11103886409125155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT139800.018.0200921
CGGACTG129150.017.7043041
CTTTGCG135450.017.4858681
GCGGGTT139450.017.3543171
GACTAAC117250.017.2145181
GGGAATG131250.017.2051981
GGGACCT114750.017.1046371
TGACTAG132450.017.0722351
TGGCTGG159650.016.9772991
CTGATAG154500.016.9669481
GTGATAA138400.016.88098
ACTGATG109450.016.8144151
TGGCCAG139350.016.778681
ATGCGAT107650.016.7666241
CGGACGT146450.016.752791
GATCGCG112150.016.6712271
GGTATTG141250.016.64611
CGTCTAC145050.016.642461
CTCTACG131900.016.6218341
CTAGCCT128000.016.614511
Produced by FastQC (version 0.11.5)