FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33535124
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAAGTTTCAGCTTTGCAACCAT719970.21469131886913553No Hit
TGCCCTCCAATGGATCCTCGTTAAAG630510.18801481097848335No Hit
CGTTCGTTATCGGAATTAACCAGACA600690.17912264168159928No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA340840.10163671975687341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG120300.017.8604051
GAATGAA111250.017.5427061
CCTTACG135950.017.5208051
ATGCGAT85200.017.4643571
GATCGCG85400.017.1277451
GGCTCGA119550.017.1274871
GGAACTT117650.017.0692311
GATGAGG159900.017.0381321
GAACCTA95750.016.9432321
CCGGGAT81900.016.9223121
CTGCCTA103750.016.8440881
ACTGATG81400.016.840111
CCGAATT101600.016.81242810
TAAGTGC175450.016.8119831
CACCGAA83000.016.810848
GGACATT232650.016.7903821
TTAACTC120250.016.775771
CTGTGCT160200.016.7287791
CGGAGCT76800.016.7044431
GGGATGA109400.016.6943911
Produced by FastQC (version 0.11.5)