FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49363262
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT164850.017.8407211
ACTGATG166950.017.598131
GACTAAC132400.017.2082521
GATGCTA151300.017.0847361
TGACAAC165000.017.0457921
GCGCGAT114200.017.0006371
ATAACGC148550.016.9651371
CAATGGA178550.016.9230048
AGTGAGG199300.016.8127751
TGAGCCG143700.016.791441
ACTGAAC124200.016.6826361
CTGATCC180700.016.5893671
CCGGGAT112400.016.4177971
GGGTTGC144950.016.4163061
CGAATGT160500.016.4079931
GATGAAA135900.016.392841
TGGTTAG177950.016.3909051
CGCTATC148750.016.3233991
TCCCGAT125950.016.3062531
CTGATAG159050.016.296781
Produced by FastQC (version 0.11.5)