FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38784481
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA417810.10772607734521444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC109500.017.6313781
GGCGACT123700.017.4737721
CGGACTG116450.017.4448451
GGGAATG122250.017.3594041
GCGGGTT117750.017.295061
GGGACCT100800.017.292831
CTTTGCG123900.017.2584171
TGACTAG120100.017.0741671
ACTGATG96650.017.0444031
TGGCTGG145950.017.0410611
CTGATAG141950.016.9317741
CGTCTAC129500.016.8935761
GTGATAA120800.016.879178
CTCGAGG104150.016.8430821
CGGACGT133850.016.8341331
ATGCGAT97100.016.7889061
GATCGCG104450.016.6981851
CCCGAAA118450.016.67399610
TGACGGC97650.016.6736951
GGGAGAT78500.016.6443541
Produced by FastQC (version 0.11.5)