FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35156229
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAAGTTTCAGCTTTGCAACCAT736100.2093796806250181No Hit
TGCCCTCCAATGGATCCTCGTTAAAG686290.19521149438411042No Hit
CGTTCGTTATCGGAATTAACCAGACA629240.1789839291352892No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA355780.1011997048944015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG131450.017.8830031
ATGCGAT91150.017.4209251
CCTTACG144350.017.3292141
GATCGCG87200.017.2419341
CCGGGAT81950.017.1078741
GAATGAA114950.017.066391
GGGATGA120050.017.0613271
GATGAGG170500.016.9112991
TTAACTC121100.016.88851
CACCGAA88000.016.8460758
GGCTCGA124800.016.7985021
GGACATT244900.016.7966921
CTGCCTA110700.016.7955761
TCTTGAG108200.016.7775638
TAAGTGC191050.016.770351
GAACCTA106750.016.7674431
CTGTGCT169550.016.7630271
CGTGAGC83250.016.6596411
GCGGGTT184050.016.6491641
CAATGGA215400.016.6048498
Produced by FastQC (version 0.11.5)