FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17452928
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAAGTTTCAGCTTTGCAACCAT365590.2094720152400789No Hit
TGCCCTCCAATGGATCCTCGTTAAAG337230.19322259279359888No Hit
CGTTCGTTATCGGAATTAACCAGACA309790.17750030252803428No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA175240.10040722106915241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG65300.017.7828941
ATGCGAT44750.017.6598171
GAATGAA57200.017.6401061
CCTTACG73100.017.3608721
GGGATGA60100.017.2753121
GATGAGG84600.017.1218051
CCGGGAT40650.017.1100671
GATCGCG44350.017.0235961
CCGAATT54500.017.00202210
CTGCCTA53900.016.8873751
CCTCTGA62400.016.8324091
CACCGAA45800.016.8051578
TCTTGAG51550.016.7921588
GGACATT122700.016.7918851
GGGACCT39700.016.7577821
GAACCTA49900.016.7462331
TTAACTC61300.016.7233621
CGGAGCT39300.016.7231541
TAAGTGC92000.016.697841
GCGGGTT93250.016.5712971
Produced by FastQC (version 0.11.5)