FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24010981
Sequences flagged as poor quality0
Sequence length98
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT319400.13302247001070053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88700.042.5675669
GTGGTAT107400.034.2565846
GGTATCA110200.033.970518
ATCAACG79100.030.0306610-11
AGTGGTA134350.028.137315
TCAACGC88350.025.04016312-13
CAGTGGT159250.023.88414
TGGTATC157450.023.2503137
AGAGTAC95900.021.2940818-19
ACGCAGA113650.021.12606814-15
AGCAGTG219250.018.822832
CAACGCA133900.018.35970712-13
AAGCAGT259400.016.5758021
TATCAAC123800.016.23423610-11
GTACTTT109500.015.85643422-23
AACGCAG150650.015.1283714-15
GCAGAGT153400.014.07767916-17
CGCAGAG183000.012.07711116-17
GCAGTGG358300.012.0275053
CTACACG16700.011.1338741
Produced by FastQC (version 0.11.5)