FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45427972
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC17141743.7733887834570297No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT13198762.905425758385164No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG9283612.0435889147770014Clontech Universal Primer Mix Short (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCTCGTAT5727481.2607826737235817TruSeq Adapter, Index 7 (97% over 35bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG4925511.0842460675990555No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCGCGTAT3892820.8569213699436109TruSeq Adapter, Index 7 (97% over 35bp)
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC2797060.615713155762269No Hit
AGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCC1790450.3941294143617065No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA1719450.3785002773181246No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA1672980.3682708970587549No Hit
ACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCG1672640.3681960533038983No Hit
TACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTC1615390.3555936857581932No Hit
AATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTC1592710.35060116705187716No Hit
AATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGG1286830.28326820312383744Clontech Universal Primer Mix Short (95% over 21bp)
TAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCT1202330.2646673287550675No Hit
ATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGC1159900.2553272684063467Clontech Universal Primer Mix Short (95% over 21bp)
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA1041890.22934988161038752No Hit
GACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCC992900.21856577705031605No Hit
ACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCA835580.1839351314207907No Hit
GAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAG795270.17506174389646978No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG762050.1677490687895995No Hit
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC686510.1511205474899914No Hit
GAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCG678020.14925165490548423No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA489540.10776179927204323No Hit
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA460720.10141769040449351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)