FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005407697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45427972
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC23302935.129643471647821No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT7832151.724080925294222No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG5956341.3111613258896964Clontech Universal Primer Mix Short (95% over 21bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG4328360.9527962199149018No Hit
AGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCC3134790.6900572184908452No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC2597890.5718701244246606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1752860.38585477687623826No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA1637830.3605333735787281No Hit
ACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCG1149940.2531347866464301No Hit
TACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTC1122010.24698659231365203No Hit
AATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTC990830.21811011066045385No Hit
AATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGG887470.19535760918405073Clontech Universal Primer Mix Short (95% over 21bp)
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC883960.19458495747950183No Hit
GAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAG857230.1887009175756294No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA854670.18813738812729744No Hit
TAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCT713650.15709484015707326No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG661040.14551386973646985No Hit
ACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCA647400.1425113143945761No Hit
ATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGC634620.13969806972673138Clontech Universal Primer Mix Short (95% over 21bp)
GAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCG616180.13563889666921516No Hit
GACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCC608740.1340011392100004No Hit
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA547820.12059089936922564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)