FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3862155
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT115830.29991028324860086No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGAGCTATCTCGTAT46040.11920805871333492TruSeq Adapter, Index 7 (97% over 36bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC45150.11690364576253413No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT39840.10315484489876767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)