FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005407706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53726900
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCCAGATCTCGTAT3972240.7393391392393754TruSeq Adapter, Index 7 (97% over 35bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC2729410.5080155378404487No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCCAGATCGCGTAT2256150.4199293091542598TruSeq Adapter, Index 7 (97% over 35bp)
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT2055460.3825755813195997No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1508010.28068062739521543No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1445770.2690961138647493No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG1184620.22048917767449827Clontech Universal Primer Mix Short (95% over 21bp)
CCCAGACCCGCCGCGACGCCGCCGAGAACCGCCCCCGCGCCCGCCGACAC1029690.19165259860516798No Hit
GCGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGG996780.18552717540003238No Hit
CCCGGAACCGTCTCCTCCCCCGCACGCGCCGCAGGCCGACCCCCGGAACC849270.1580716549810244No Hit
CCGGAACCGTCTCCTCCCCCGCACGCGCCGCAGGCCGACCCCCGGAACCC841820.15668501253561998No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG829210.15433795733608305No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA817010.15206721400266907No Hit
GGCGAGGCCGGGGCGACGGGGAAGGCGCGAGAAAGGCGGCCGGCGGGGAA772540.14379016842587233No Hit
GGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGC765510.1424816991116182No Hit
GCCGGGGCGACGGGGAAGGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGAC709690.13209211772873553No Hit
GCGACGGGGAAGGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACG696480.12963338662755527No Hit
GGCGGCACCGCGCGGCACCCGGGGCGGCCGACCGGCCCTCGGCGATCCCC688510.12814995840072663No Hit
GGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCG683060.1271355689607999No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA647470.12051132672832418No Hit
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG618990.11521044393032168No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC604360.11248741319525228No Hit
CGGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCC577990.10757925731802877No Hit
GCCGGCGGCACCGCGCGGCACCCGGGGCGGCCGACCGGCCCTCGGCGATC567330.10559514879883262No Hit
GGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGG542220.10092151231506005No Hit
GGGGAAGGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGAC537510.10004485648716006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)