FastQCFastQC Report
Thu 12 May 2022
EGAF00005407710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32129870
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTCTTCCACGGCTCTCAAGGGAGTTCTCGTTTCAAGGGGGGTTTCCC2046190.6368497600519392No Hit
GTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACT1198200.37292401120826196No Hit
CTCGCAGTTCCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGG1085730.337919201042519No Hit
GGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAA1060420.3300417959985521No Hit
CACGAATTGGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGA1016820.31647186870037136No Hit
GCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAAC971850.3024755468976376No Hit
GCGGTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCCAT879790.2738230811391394No Hit
CGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCG750440.23356459269832092No Hit
CCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCC745760.23210800417181893No Hit
GTCGTCTTCCACGGCTCTCGAGGGAGTTCTCGTTTCAAGGGGGGTTTCCC734280.2285350049657842No Hit
GCACGAAGTTAGCCGGGGCTTATTCCTCCGGTACCGTCATTATCGTCCCG730740.22743322646496858No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCT714630.22241920057566372No Hit
GTCGGTTTCGGGTACGGTCTGATGTGGAGGCTATTTCCTGGGACCCCTTC712660.22180606395232846No Hit
CTCGTTTCAAGGGGGGTTTCCCGCTTAGATGCCTTCAGCGGTTATCCCGA688410.214258569984877No Hit
CCCAGGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACC667560.20776928135719194No Hit
GGGAGTTCTCGTTTCAAGGGGGGTTTCCCGCTTAGATGCCTTCAGCGGTT657980.20478763219396778No Hit
CTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTGATCGTCG641460.19964599919016168No Hit
CGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGT624940.19450436618635555No Hit
CTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC613150.19083488355228329No Hit
CACGTGTGTAGCCCATCCCGTAAGGGCCATGAGGACTTGACGTCATCCAC523930.1630663304893546No Hit
GCCACGAATTGGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCAC518680.16143233695000947No Hit
CACGAAGTTAGCCGGGGCTTATTCCTCCGGTACCGTCATTATCGTCCCGG511920.15932837574506215No Hit
GCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTG498460.15513912754704579No Hit
CCAGGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCC498120.15503330701306914No Hit
GTCGGTTTCGGGTACGGTCTATACGGTGGGGCTATTTCCCGGGACAGCTT491190.15287643554113353No Hit
GGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTA479810.14933456002156248No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCAC474710.1477472520119129No Hit
GGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGACGGACGTT473670.14742356567269024No Hit
CACGTGTGTAGCCCAGCGCGTAAGGGCCATGAGGACTTGACGTCATCCCC420480.13086887684263895No Hit
GGGGGTTTCCCGCTTAGATGCCTTCAGCGGTTATCCCGACCGAACATAGC418810.1303491112786949No Hit
CAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGC416120.12951188411282089No Hit
CTGGTCTGTGCCCCCTGCCGATGCTTGCGCACCCACAGGGCCTCCTTATC406590.12654579679282862No Hit
CTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGACGGACGTTAGCACC405310.12614741360609302No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATC404990.12604781780940913No Hit
CCCGGATTCAAGCGATGCAGTCTCAAAGGCAGTTCTGGAGTTGAGCTCCA391070.12171540065365966No Hit
CTGGTTTCGGGTCTAGAGCCACGAACTGAACGCCCTGTTCAGACTCGCTT390660.12158779353915843No Hit
CTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG389330.12117384850919097No Hit
GGAATATTCGCCCCATTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG389310.12116762377189824No Hit
CCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGGCCATTCCGT388110.12079413953433363No Hit
GTGAAATCTTATCTCGAGGGAGGCTTCCCGCTTAGATGCTTTCAGCGGTT388020.12076612821651628No Hit
GTGCGGTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCC385050.11984175472854387No Hit
GGATAACTTAATGCGTTAGCTGCGCCACCCAAGCACCAAGTGCCCGGACA382620.11908544914747556No Hit
CCTGGTTTCGGGTCTAGAGCCACGAACTGAACGCCCTGTTCAGACTCGCT374900.11668270055247656No Hit
AGCGGCGCCACTGAGGTGCATGCACCCCAACGGCTAGCATTCATCGTTTA368910.1148183917332999No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT368310.11463164961451758No Hit
GTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGGCCATTCCGTGGTTG362950.11296342002006232No Hit
CCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTGATCGTCGCCTTGG361940.11264907078677877No Hit
GTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCCATCCA360230.11211685574824921No Hit
CAGGGTTGTTGCCCTCTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC354420.11030856956470722No Hit
GGGGAATTCAATTTCACTGAGCCGATGCTGGAGACAGCGGGGAGATCGTT349500.10877728419069234No Hit
CTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCCATGAG346530.10785291070271993No Hit
GCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAACGGCTAGCATTCATC344580.10724599881667743No Hit
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCA334210.10401847253038994No Hit
CTCTACACTCGCAGTTCCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTA324940.10113330679520334No Hit
TGCGGTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCCA324040.10085319361702988No Hit
CCGACATCGAGGTGCCAAACGACCCCGTCGATATGGACTCTTGGGGGTCA323630.10072558650252865No Hit
GTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCCATGAGAGCT323170.10058241754479555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)