FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005407711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32129870
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGAAACTTGAGCT1264730.39363059981257315No Hit
GGCCAATCAAACCTGGACATAGCTGGTTCTCCGCGAAAGCTATTTAGGTA1052710.3276421597721995No Hit
GGCGAATATTCCCCAGCCAGTGGATGGTGACGGATGCCGTGTATCGTCAG898580.2796712218256719No Hit
AGAGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCTCTGGTG881310.2742961611733879No Hit
CTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGCTGATTTAGTCGAGG818010.25459486764185474No Hit
CCGGTTCTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGAT767090.23874668649452985No Hit
GAGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCTCTGGTGG551030.17150084952102204No Hit
GCTACACACGTGCTACAATGGCGGTGACAATGGGCAGCGAAGGGGCGACC526630.163906670023875No Hit
GGGAAACTTGAGCTAATACCGGATACGTGCGAGAGCAGAAAGGTTTATCT515760.1605235253052689No Hit
CGAAATTTGAGAGGAGTTGACCCTAGTACGAGAGGACCGGGTTGAACGTA496730.15460068777122346No Hit
CCCTGATCCAGCCATGCCGCGTGAGTGATGACGGCCTTAGGGTTGTAAAG482210.1500815284966917No Hit
CTGGGCGTAAAGGGCGCGTAGGCGGCTGATTTAGTCGAGGGTGAAAGCCC469490.14612259557850685No Hit
CAAACCTGGACATAGCTGGTTCTCCGCGAAAGCTATTTAGGTAGCGCCTC462050.14380699330560626No Hit
CGAACCTCTGGTGGACCTGTTGTGGCGCCAGCCGCAGTGCAGGGTAGCTA461740.14371050987756875No Hit
CAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACC459190.14291685587274394No Hit
GGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGAGTGATGACGGC456540.14209207818145544No Hit
GCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTT450200.14011883645965575No Hit
GGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGAGTG445950.1387960797849478No Hit
GAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTAGGC443220.13794640314448828No Hit
CGGGTGAGTAACGCGTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGG439040.1366454330503049No Hit
CGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAG435660.13559345244783125No Hit
GGAAACTTGAGCTAATACCGGATACGTGCGAGAGCAGAAAGGTTTATCTG429210.13358597467092148No Hit
GGTTAAGTCCCGCAACGAGCGCAACCCACGTCCTCAGTTGCCATCATTCA424530.13212938614441952No Hit
GTTTAATTCGAAGCAACGCGCAGAACCTTACCATCCCTTGACATGGCATG421680.13124236108020354No Hit
CACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTA417300.12987914361309275No Hit
GGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGC416340.12958035622304107No Hit
GTTCTGAGAGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCT410990.12791523899723217No Hit
GGGTGAGTAACGCGTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGA406780.12660493179710966No Hit
AGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCTCTGGTGGA404540.1259077612203224No Hit
GGCAGTTTGACTGGGGCGGTCGCCTCCCAAAGCGTAACGGAGGCGTGCAA404500.1258953117457369No Hit
GCCAATCAAACCTGGACATAGCTGGTTCTCCGCGAAAGCTATTTAGGTAG402530.12528217512240167No Hit
CGGAAATCGGTCGTCGAGTGCAATGGCATAAGCCTGCCTGACTGCGAGAC398250.12395008134175457No Hit
CTTGAATTGAAGCCCCGGTAAACGGCGGCCGTAACAATAACGGTCCTAAG383600.11939046127481998No Hit
GTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGAAACTTGAGCTAAT373460.11623451946739902No Hit
GCCGGGGATAACAGGCTGATGACCCCCAAGAGTCCATATCGACGGGGTCG355000.1104890869461968No Hit
TGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGAAACTTGAGCTAATA354790.11042372720462298No Hit
CGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGCTGATTTAGT346930.10797740544857481No Hit
CTAAAACACAGGACTCTGCGAAGTCGAGAAGACGACGTATAGGGTCTGAC340060.10583920818851743No Hit
CCGGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGAT338740.10542837552719635No Hit
CCGGTGGTCCCGCGTGGGTGGGCCATCGCTCAACGGATAAAAGGTACGCC336530.1047405420563482No Hit
CGGGGCTCAAGCCACGAGCCGAAGCTTCGGGTTCATCGCAAGATGAGCGG329670.10260545716493717No Hit
TTTAATTCGAAGCAACGCGCAGAACCTTACCATCCCTTGACATGGCATGC321530.10007198908679059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)