FastQCFastQC Report
Thu 12 May 2022
EGAF00005407712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35076371
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTCTTCCACGGCTCTCAAGGGAGTTCTCGTTTCAAGGGGGGTTTCCC2106030.6004127393908566No Hit
CTCGCAGTTCCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGG1265210.36070151042706217No Hit
GTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACT1238300.35302967915352473No Hit
GGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAA1144130.3261825460792395No Hit
GCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAAC1045280.29800118147912164No Hit
CACGAATTGGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGA974310.2777681875927245No Hit
CCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCC787440.22449300698752445No Hit
CGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCG787020.22437326826084714No Hit
GCGGTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCCAT779170.22213529444080746No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCT757910.2160742341332859No Hit
CTCGTTTCAAGGGGGGTTTCCCGCTTAGATGCCTTCAGCGGTTATCCCGA751040.21411565067549318No Hit
GTCGTCTTCCACGGCTCTCGAGGGAGTTCTCGTTTCAAGGGGGGTTTCCC748550.21340577108162076No Hit
GCACGAAGTTAGCCGGGGCTTATTCCTCCGGTACCGTCATTATCGTCCCG726100.20700545104851356No Hit
CCCAGGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACC711410.20281744653687234No Hit
CTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTGATCGTCG689750.196642349346801No Hit
GGGAGTTCTCGTTTCAAGGGGGGTTTCCCGCTTAGATGCCTTCAGCGGTT677900.19326400670126337No Hit
GTCGGTTTCGGGTACGGTCTGATGTGGAGGCTATTTCCTGGGACCCCTTC640360.1825616452739652No Hit
CGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGT622200.17738437080620456No Hit
CTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC610630.17408585397845178No Hit
CCAGGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCC532410.15178594159583955No Hit
CACGAAGTTAGCCGGGGCTTATTCCTCCGGTACCGTCATTATCGTCCCGG521450.1486613310139752No Hit
GCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTG519870.1482108853279035No Hit
CACGTGTGTAGCCCATCCCGTAAGGGCCATGAGGACTTGACGTCATCCAC519830.14819948163964852No Hit
GCCACGAATTGGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCAC490080.1397179885000076No Hit
GGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTA487890.1390936365680475No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCAC476720.13590915662284447No Hit
GTCGGTTTCGGGTACGGTCTATACGGTGGGGCTATTTCCCGGGACAGCTT471980.13455781956462942No Hit
GGGGGTTTCCCGCTTAGATGCCTTCAGCGGTTATCCCGACCGAACATAGC462630.13189220743502797No Hit
GGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGACGGACGTT447980.1277156066116418No Hit
CCCGGATTCAAGCGATGCAGTCTCAAAGGCAGTTCTGGAGTTGAGCTCCA442510.12615615224277335No Hit
CAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGC439850.12539780697381722No Hit
CCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGGCCATTCCGT427070.12175432857635131No Hit
GGATAACTTAATGCGTTAGCTGCGCCACCCAAGCACCAAGTGCCCGGACA426050.12146353452584932No Hit
CACGTGTGTAGCCCAGCGCGTAAGGGCCATGAGGACTTGACGTCATCCCC422440.12043435166083745No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATC418750.11938236141931559No Hit
GTGAAATCTTATCTCGAGGGAGGCTTCCCGCTTAGATGCTTTCAGCGGTT415940.11858125231940328No Hit
CTGGTTTCGGGTCTAGAGCCACGAACTGAACGCCCTGTTCAGACTCGCTT402540.11476101675398519No Hit
GTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGGCCATTCCGTGGTTG400100.11406539177043144No Hit
CTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG392900.11201272788453513No Hit
AGCGGCGCCACTGAGGTGCATGCACCCCAACGGCTAGCATTCATCGTTTA391060.11148815822480611No Hit
GGAATATTCGCCCCATTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG390870.11143399070559494No Hit
CTGGTCTGTGCCCCCTGCCGATGCTTGCGCACCCACAGGGCCTCCTTATC388960.11088946459141967No Hit
CCTGGTTTCGGGTCTAGAGCCACGAACTGAACGCCCTGTTCAGACTCGCT380640.10851749743438398No Hit
CCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTGATCGTCGCCTTGG379400.1081639830984796No Hit
CTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGACGGACGTTAGCACC378930.10802998976148358No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT370320.10557534586459927No Hit
CTCTACACTCGCAGTTCCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTA361440.10304372707199386No Hit
GGGGAATTCAATTTCACTGAGCCGATGCTGGAGACAGCGGGGAGATCGTT357100.10180642689632859No Hit
GCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAACGGCTAGCATTCATC355320.101298962768982No Hit
GTGCGGTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCC351900.10032394742318126No Hit
CTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCCATGAG351270.10014433933316534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)