FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19071310
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTCTTCCACGGCTCTCAAGGGAGTTCTCGTTTCAAGGGGGGTTTCCC1187550.6226892646598478No Hit
GTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACT710170.37237609791881104No Hit
CTCGCAGTTCCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGG684440.35888462827147166No Hit
GGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAA633950.3324103063711932No Hit
CACGAATTGGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGA587090.30783936709119614No Hit
GCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAAC575630.3018303409676629No Hit
GCGGTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCCAT497460.2608420711529517No Hit
CGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCG444180.23290481880898586No Hit
CCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCC443960.23278946228654454No Hit
GTCGTCTTCCACGGCTCTCGAGGGAGTTCTCGTTTCAAGGGGGGTTTCCC429090.22499241006517118No Hit
GCACGAAGTTAGCCGGGGCTTATTCCTCCGGTACCGTCATTATCGTCCCG427150.22397517527637062No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCT423640.22213471439560262No Hit
CTCGTTTCAAGGGGGGTTTCCCGCTTAGATGCCTTCAGCGGTTATCCCGA405810.21278559259956448No Hit
GTCGGTTTCGGGTACGGTCTGATGTGGAGGCTATTTCCTGGGACCCCTTC405580.21266499259883037No Hit
CCCAGGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACC397040.2081870621367908No Hit
GGGAGTTCTCGTTTCAAGGGGGGTTTCCCGCTTAGATGCCTTCAGCGGTT384410.20156454905300158No Hit
CTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTGATCGTCG370440.19423940987797902No Hit
CGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGT363540.1906214098559564No Hit
CTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC356160.18675172287588004No Hit
CACGTGTGTAGCCCATCCCGTAAGGGCCATGAGGACTTGACGTCATCCAC304720.15977927053778684No Hit
CACGAAGTTAGCCGGGGCTTATTCCTCCGGTACCGTCATTATCGTCCCGG302290.15850510531263975No Hit
CCAGGCGGAATGCTTAAAGCGTTAGCGGCGCCACTGAGGTGCATGCACCC296260.15534328790208957No Hit
GCCACGAATTGGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCAC295420.1549028357254955No Hit
GCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTG287790.1509020617880995No Hit
GGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTA284580.1492189052561151No Hit
GTCGGTTTCGGGTACGGTCTATACGGTGGGGCTATTTCCCGGGACAGCTT279400.1465027835004517No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCAC274050.143697522613811No Hit
GGGGGCCTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGACGGACGTT270490.14183084434157905No Hit
GGGGGTTTCCCGCTTAGATGCCTTCAGCGGTTATCCCGACCGAACATAGC246950.1294876964403599No Hit
CAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGC246660.12933563556986907No Hit
CTGGTCTGTGCCCCCTGCCGATGCTTGCGCACCCACAGGGCCTCCTTATC242260.12702850512104308No Hit
CACGTGTGTAGCCCAGCGCGTAAGGGCCATGAGGACTTGACGTCATCCCC242090.1269393659900657No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATC236060.12377754857951552No Hit
CCCGGATTCAAGCGATGCAGTCTCAAAGGCAGTTCTGGAGTTGAGCTCCA235510.12348915727341227No Hit
CCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGGCCATTCCGT235180.12331612248975031No Hit
CTTAGCTGTCGGTCAGGGTTGTTGCCCTCTTCACGACGGACGTTAGCACC233430.12239851378851269No Hit
CTGGTTTCGGGTCTAGAGCCACGAACTGAACGCCCTGTTCAGACTCGCTT231610.12144420073922557No Hit
GGATAACTTAATGCGTTAGCTGCGCCACCCAAGCACCAAGTGCCCGGACA230660.12094607030141086No Hit
GGAATATTCGCCCCATTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG228860.12000224420870931No Hit
GTGAAATCTTATCTCGAGGGAGGCTTCCCGCTTAGATGCTTTCAGCGGTT224950.11795204419622984No Hit
CTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG223010.11693480940742929No Hit
GTGCGGTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCC219950.11533030504983664No Hit
AGCGGCGCCACTGAGGTGCATGCACCCCAACGGCTAGCATTCATCGTTTA219450.11506813113519733No Hit
CCTGGTTTCGGGTCTAGAGCCACGAACTGAACGCCCTGTTCAGACTCGCT218770.11471157461128785No Hit
GTCCTCTTCCGGTCTCAAGCCAATCAGTATCGAAGGCCATTCCGTGGTTG217230.11390407895419874No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT211110.11069507023901348No Hit
CCCAGTGTGGCTGATCATCCTCTCAGACCAGCTACTGATCGTCGCCTTGG208500.10932652240459623No Hit
CAGGGTTGTTGCCCTCTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC205020.10750179195870654No Hit
GGGGAATTCAATTTCACTGAGCCGATGCTGGAGACAGCGGGGAGATCGTT204890.10743362674090033No Hit
GCGTTAGCGGCGCCACTGAGGTGCATGCACCCCAACGGCTAGCATTCATC202530.10619616586380275No Hit
GTTAGACCCTCATAACGTCAAGGGTGGTATTTCAAGGACGGCTCCATCCA202100.10597069629721294No Hit
CTCTACACTCGCAGTTCCGCTGTCCTCTTCCGGTCTCAAGCCAATCAGTA201520.10566657455623132No Hit
CTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCCATGAG200500.10513173977036712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTCGTAT199920.1048276180293855TruSeq Adapter, Index 13 (97% over 37bp)
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCA196330.10294520932227519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)