FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19071310
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGAAACTTGAGCT736420.38614022843737533No Hit
GGCCAATCAAACCTGGACATAGCTGGTTCTCCGCGAAAGCTATTTAGGTA617250.3236536976222399No Hit
GGCGAATATTCCCCAGCCAGTGGATGGTGACGGATGCCGTGTATCGTCAG533490.2797343234418611No Hit
AGAGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCTCTGGTG526350.2759904799408116No Hit
CTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGCTGATTTAGTCGAGG496640.2604121059329432No Hit
CCGGTTCTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGAT470800.2468629580243832No Hit
GAGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCTCTGGTGG326300.1710946966936199No Hit
GCTACACACGTGCTACAATGGCGGTGACAATGGGCAGCGAAGGGGCGACC315090.16521675752740636No Hit
GGGAAACTTGAGCTAATACCGGATACGTGCGAGAGCAGAAAGGTTTATCT304340.15958001836266097No Hit
CGAAATTTGAGAGGAGTTGACCCTAGTACGAGAGGACCGGGTTGAACGTA294990.1546773661589057No Hit
CCCTGATCCAGCCATGCCGCGTGAGTGATGACGGCCTTAGGGTTGTAAAG290270.15220244440471054No Hit
CGAACCTCTGGTGGACCTGTTGTGGCGCCAGCCGCAGTGCAGGGTAGCTA286160.1500473748263753No Hit
CTGGGCGTAAAGGGCGCGTAGGCGGCTGATTTAGTCGAGGGTGAAAGCCC277750.14563760958214197No Hit
GGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGAGTGATGACGGC277300.14540165305896657No Hit
GAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTAGGC270830.1420091226035338No Hit
CAAACCTGGACATAGCTGGTTCTCCGCGAAAGCTATTTAGGTAGCGCCTC270260.14171024434084498No Hit
GGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGAGTG268130.14059338346448147No Hit
GCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTT265450.1391881312820147No Hit
CGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAG264940.13892071388908261No Hit
CAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACC263590.13821284431955644No Hit
CGGGTGAGTAACGCGTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGG255200.13381356603190867No Hit
GGTTAAGTCCCGCAACGAGCGCAACCCACGTCCTCAGTTGCCATCATTCA254840.13362480081336836No Hit
GGAAACTTGAGCTAATACCGGATACGTGCGAGAGCAGAAAGGTTTATCTG250230.13120755732039383No Hit
GTTTAATTCGAAGCAACGCGCAGAACCTTACCATCCCTTGACATGGCATG248830.13047347035940374No Hit
GGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGC246220.12910492252498648No Hit
CGGAAATCGGTCGTCGAGTGCAATGGCATAAGCCTGCCTGACTGCGAGAC243810.12784124425642496No Hit
CACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTA241430.1265932964227418No Hit
GTTCTGAGAGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCT239060.1253505920673514No Hit
GGGTGAGTAACGCGTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGA238320.12496257467368524No Hit
GGCAGTTTGACTGGGGCGGTCGCCTCCCAAAGCGTAACGGAGGCGTGCAA236500.12400826162439813No Hit
AGGATCTGTCCCTAGTACGAGAGGACCGGGATGGACGAACCTCTGGTGGA235830.12365694857878143No Hit
GCCAATCAAACCTGGACATAGCTGGTTCTCCGCGAAAGCTATTTAGGTAG235570.12352061814316898No Hit
CTTGAATTGAAGCCCCGGTAAACGGCGGCCGTAACAATAACGGTCCTAAG226650.11884343550600351No Hit
GTGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGAAACTTGAGCTAAT220900.11582843548765134No Hit
GCCGGGGATAACAGGCTGATGACCCCCAAGAGTCCATATCGACGGGGTCG209900.11006060936558631No Hit
CGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGCTGATTTAGT207820.10896996588068675No Hit
TGGGAACGTGCCCTTCGGTTCGGAATAACTCAGGGAAACTTGAGCTAATA207800.10895947892410117No Hit
CCGGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGAT204160.10705085282552694No Hit
CTAAAACACAGGACTCTGCGAAGTCGAGAAGACGACGTATAGGGTCTGAC202420.1061384876025821No Hit
CGGGGCTCAAGCCACGAGCCGAAGCTTCGGGTTCATCGCAAGATGAGCGG199080.10438716585279145No Hit
CCGGTGGTCCCGCGTGGGTGGGCCATCGCTCAACGGATAAAAGGTACGCC198290.10397293106766133No Hit
GACGAACCTCTGGTGGACCTGTTGTGGCGCCAGCCGCAGTGCAGGGTAGC194750.10211673975201493No Hit
CAGGAAATAGCCTCCACATCAGACCGTACCCGAAACCGACACAGGTGGAC191590.10045980061149445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)