FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005407737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59698164
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC23890294.001846689958506No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT8002611.340511912560661No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG5730890.9599775966309451Clontech Universal Primer Mix Short (95% over 21bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG4582400.7675947957126453No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC3243140.5432562381650464No Hit
AGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCC3068660.514029208670471No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA1701930.2850891695764714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1572840.26346538898583216No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA1287810.2157202020484248No Hit
TACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTC1202500.2014299803256931No Hit
ACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCG1134990.1901214248397991No Hit
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC1002510.1679297875894475No Hit
GAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAG925980.15511029786443684No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG917090.15362113983940945No Hit
AATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTC844600.1414783878445575No Hit
AATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGG813230.13622361987547893Clontech Universal Primer Mix Short (95% over 21bp)
ACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCA775560.12991354306976677No Hit
GACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCC718670.1203839367656265No Hit
TAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCT713480.1195145632954474No Hit
GAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCG681530.11416263990966288No Hit
ATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGC608710.10196460983289202Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)