FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005407792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59173008
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTAT2216610.3745981613779039TruSeq Adapter, Index 15 (97% over 36bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA2026670.34249906646625095No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1891460.3196491210992688No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCGCGTAT1186660.20054076007087557TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTTT700600.11839857794621493TruSeq Adapter, Index 15 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)