FastQCFastQC Report
Wed 11 May 2022
EGAF00005407822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27657885
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1040510.3762073636505467No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1015140.3670345726001826No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC959440.34689565019161805No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA684910.24763643351615644No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC498350.18018369806657306No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT469640.16980329479278694No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC459210.1660322182986877Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA450650.16293726002548642No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT446120.16129939075240207No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT419220.1515734120667578No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA392030.14174258082279248No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC382320.13823182792176625Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC367450.13285542260371683No Hit
CTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAA367150.1327469544399364No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC340620.12315475315628797No Hit
CTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACT293700.10619033234103041No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA282450.10212277619926469No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)