FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38562801
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1398130.3625592445942918No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1389950.3604380293848468No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1298720.3367805155024916No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA946480.24543860286497343No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC753130.1952996101087159Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC683150.17715258806018785No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT645520.16739447946221542No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA635550.16480908635241512No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC626470.16245448560647865Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT615450.1595968093707716No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT600590.15574335484603413No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA570910.14804681848706996No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC510020.13225699035710606No Hit
CTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAA498590.129292994043664No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC458730.11895660794971818No Hit
CTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACT416990.10813270540176788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)