FastQCFastQC Report
Thu 12 May 2022
EGAF00005407825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38562801
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA991370.257079354790644No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG709220.1839129891005583Illumina Single End PCR Primer 1 (100% over 32bp)
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA656560.17025734204317783No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA598820.15528436329093417Illumina Single End PCR Primer 1 (100% over 34bp)
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT463120.12009501073327117No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG449220.11649050078079132No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC441180.11440558998813391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)