FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9161041
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC484010.5283351531774609No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT378620.41329364206535046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACATCTCGTAT247860.2705587716505144TruSeq Adapter, Index 15 (97% over 36bp)
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG227950.24882543370344049Clontech Universal Primer Mix Short (95% over 21bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG153360.1674045558796211No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACATCGCGTAT117630.12840243810719765TruSeq Adapter, Index 15 (97% over 36bp)
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC103330.11279285836620533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)