FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9161041
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC637110.6954558985163367No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT229540.25056104431799836No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG146050.1594251133686663Clontech Universal Primer Mix Short (95% over 21bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG131230.14324791254618333No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC98850.10790258443336298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)