FastQCFastQC Report
Sun 15 May 2022
EGAF00005407836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61248431
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC4250590.6939916550678662No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT4187930.6837611889192721No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGCTAATCTCGTAT3207700.5237195382196811TruSeq Adapter, Index 4 (97% over 37bp)
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG2065680.3372625169777818Clontech Universal Primer Mix Short (95% over 21bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG1776830.29010212522831813No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGCTAATCGCGTAT1606290.2622581466617488TruSeq Adapter, Index 4 (97% over 37bp)
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC1497040.24442095504454636No Hit
CGGCGAATTAGTACCAGTCACCTCCACGCCTTACAACGCTTCCAGATCTA1141850.18642926542885646No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA1052380.1718215442939265No Hit
GGCGAATTAGTACCAGTCACCTCCACGCCTTACAACGCTTCCAGATCTAG976580.1594457170666135No Hit
GTGGGTTCGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGG922740.15065528780647458No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTAC900750.14706499175464594No Hit
GTTCGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGGGTAG835280.13637573834340344No Hit
GTGAAGCCCTGGACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTT746980.12195904251000324No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA684990.11183796691869544No Hit
CGGCGATCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGT674710.11015955657704929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)