FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005407837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61248431
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC5573670.9100102498952177No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT2603200.42502313242930256No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG1902130.3105597921357365No Hit
AGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCC1413710.23081570856892647No Hit
GTTGGTGTAAGCGTTGAGGTTGGTGCCATTGGTAAATCCGGGTGCTGTAT1413330.23075366616330137No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG1325880.2164757493951151No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG1271010.2075171525618346Clontech Universal Primer Mix Short (95% over 21bp)
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC964180.15742117540937497No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG892560.14572781464393758No Hit
TGTTGGTGTAAGCGTTGAGGTTGGTGCCATTGGTAAATCCGGGTGCTGTA833530.13609001673855123No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA829690.13546306190276122No Hit
GGTTAAGTCCCGCAACGAGCGCAACCCTTGTCTTATGTTGCCAGCACGTT809490.13216501823532428No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA755210.12330275040025107No Hit
GTGGAAGGCTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCG726600.11863161033463861No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA668510.10914728574843004No Hit
GCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGGGGGATGACGGC659780.1077219431139387No Hit
GGGTGATCTGCCCTGCACTCTGGGATAAGCCTGGGAAACTGGGTCTAATA655070.10695294382316504No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)