FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45692489
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1158100.2534552232424896Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG963610.21089024062576237Illumina Single End PCR Primer 1 (96% over 33bp)
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT808250.17688902874168225No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG581630.12729225584537537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)