FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005407848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29962723
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACC1036280.34585641632103997No Hit
CCCGGATCGGCCCACGAAGGACCTTGATACTAGAAGTTGGGCGGTAAAAC615020.20526171803544024No Hit
GCCGCTTACAGCCATTACGCCAGCATCCTAGCAGATGCGCGGACCTCAGT604590.20178072600410848No Hit
CTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAA524150.1749340338660141No Hit
GCGGATTTTCAAGGGTCGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAG485380.16199462245137067No Hit
CTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACAT482710.16110351519119273No Hit
GTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGT469170.15658456676317437No Hit
GAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACCAA449090.14988290616977637No Hit
CCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCC404650.13505114338239552No Hit
GCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACA392010.13083256818814498No Hit
CCCAAACTGATGCTGGCCTGCCTACAGAAGAGTGCACCGGGTAGAAACCC368560.12300617670830517No Hit
GCCCAAACTGATGCTGGCCTGCCTACAGAAGAGTGCACCGGGTAGAAACC366820.12242545512302071No Hit
CTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATTTTAAC326930.10911224590635503No Hit
CCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATT316940.10577810301153202No Hit
AAAGGATTCAATCCTGCGCACTGTACAATTGCAATACGCCGGCGAACAGC303930.10143604104339916No Hit
GTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATTTTAACCGGC303450.10127584198538965No Hit
CGCGGACCTCAGTCCAGGCTGGTAGTATGTCGTCTCCCCTATAAGGCCTC302180.10085198197773947No Hit
CTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGC300400.1002579104709542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)