FastQCFastQC Report
Thu 12 May 2022
EGAF00005407849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29962723
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAA1483760.4952019881504094No Hit
TTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAA662660.2211614745428845No Hit
CGCAACTGAGAGCGGAGACGTTGGCGGGAGCCCCAAGAAGAGTTCTCTTT657040.2192858105720231No Hit
GGGAAAGGTTCCGTGTGAACAGCAGTTGGACACGGGTCAATCGATCCTAA513110.17124945553179527No Hit
GGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTT511930.1708556328475219No Hit
GGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAG468030.15620409400040178No Hit
GGAAAGGTTCCGTGTGAACAGCAGTTGGACACGGGTCAATCGATCCTAAG385110.12852970672925823No Hit
CCGGCATCTGGATGTGGATTCTCCGCGGCAACGCAACTGAGAGCGGAGAC385020.12849966940588145No Hit
GGGAGGTACATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGC377590.12601992148710917No Hit
CACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGC325390.10859827392857452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)