FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33923911
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACC1084470.31967717401451734No Hit
CCCGGATCGGCCCACGAAGGACCTTGATACTAGAAGTTGGGCGGTAAAAC695670.20506774705310363No Hit
GCCGCTTACAGCCATTACGCCAGCATCCTAGCAGATGCGCGGACCTCAGT675570.19914272266543798No Hit
CTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAA609580.17969036647926592No Hit
CTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACAT570790.1682559537430693No Hit
GCGGATTTTCAAGGGTCGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAG516960.15238808992276862No Hit
GTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGT489070.1441667501132166No Hit
GAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACCAA468360.13806191155259193No Hit
CCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCC458910.13527626575839088No Hit
GCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACA454680.1340293576409866No Hit
CCCAAACTGATGCTGGCCTGCCTACAGAAGAGTGCACCGGGTAGAAACCC409220.12062878009554971No Hit
GCCCAAACTGATGCTGGCCTGCCTACAGAAGAGTGCACCGGGTAGAAACC405180.11943787967136218No Hit
CTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATTTTAAC367040.10819507220143339No Hit
CCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATT360410.10624069848550186No Hit
AAAGGATTCAATCCTGCGCACTGTACAATTGCAATACGCCGGCGAACAGC351640.10365550127755022No Hit
GTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATTTTAACCGGC345980.1019870615743568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)