FastQCFastQC Report
Thu 12 May 2022
EGAF00005407851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33923911
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAA1667700.4916001577766196No Hit
TTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAA745920.21988030802226782No Hit
CGCAACTGAGAGCGGAGACGTTGGCGGGAGCCCCAAGAAGAGTTCTCTTT699390.20616431873082086No Hit
GGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTT576780.17002166996606022No Hit
GGGAAAGGTTCCGTGTGAACAGCAGTTGGACACGGGTCAATCGATCCTAA569940.16800539301025758No Hit
GGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAG534440.15754079769870874No Hit
GGAAAGGTTCCGTGTGAACAGCAGTTGGACACGGGTCAATCGATCCTAAG424070.12500622348643706No Hit
GGGAGGTACATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGC418780.1234468513963499No Hit
CCGGCATCTGGATGTGGATTCTCCGCGGCAACGCAACTGAGAGCGGAGAC417910.12319039511688379No Hit
CACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGC364790.10753182320281408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)