FastQCFastQC Report
Thu 12 May 2022
EGAF00005407852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32983622
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1206670.3658391428327671No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1156600.3506588815503646No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1140660.34582618003565524No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1062420.32210531639005563Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC904450.2742118497477324Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT874860.2652407306874909No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA711810.21580710572053No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC619170.1877204389499734No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC507830.15396429173242407TruSeq Adapter, Index 5 (97% over 35bp)
CTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAA487300.1477399904716347No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA418670.1269326940503987No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT408420.12382509113159253No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG399730.12119045021799No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCA398780.12090242848405187TruSeq Adapter, Index 6 (97% over 37bp)
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC393450.11928647496627265No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC373560.11325620939992581No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA355160.107677683184703No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT341180.10343921598422394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)