FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20770666
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC773870.3725783275317219Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC758200.36503403405552814No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG743660.3580337770584727No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC657160.31638850675274444Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC654440.31507896761711923No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT536110.25810920073530624No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA460270.22159616836552087No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC381400.18362434791450596No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC375820.18093786689362776TruSeq Adapter, Index 5 (97% over 35bp)
CTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAA308130.1483486374486018No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCA294440.14175761143142931TruSeq Adapter, Index 6 (97% over 37bp)
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA271770.1308431804738471No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT258210.12431474272418612No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG251750.12120458727707625No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC242970.1169774719789919No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA237010.11410804063769549No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC232270.11182597611458389No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG225100.1083739924372189Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT221700.1067370685176874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)