FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20770666
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG751770.361938322054767Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA648700.31231545488238077Illumina Single End PCR Primer 1 (100% over 34bp)
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA610900.29411671248288335No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA428180.20614649525441311No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA369610.17794807349942462Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACC290640.13992810822724702Illumina Single End PCR Primer 1 (97% over 38bp)
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG255290.12290891394623552No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC253520.12205675061165588No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG213840.10295288557429984Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)