FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38759251
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTAT1884360.4861703854906794TruSeq Adapter, Index 22 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA1545660.39878479591878596No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT1255710.3239768487786309No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG1208570.31181459105079196No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1121360.2893141562513682Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1082190.27920818180929247Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTTT912870.23552312711099602TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGTAT821050.21183329884264276TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG741440.1912936862479618TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGAT708340.1827537895404635TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG632560.1632023281358043Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGTT567100.14631345688284844TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC515220.13292826530626198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTA499790.12894728022479074TruSeq Adapter, Index 22 (97% over 37bp)
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG481190.12414842588160437No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA463710.11963853481069589Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT450630.11626385659516485Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA409280.1055954357838339No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGAT397050.10244006005172804TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)