FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005407865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38759251
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2808660.72464248599644No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1108570.2860142988831234Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC1076970.2778614065581401Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTG740040.19093248215761446Illumina Single End PCR Primer 1 (96% over 33bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG642320.16572043665136874No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA614070.15843185411400235No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG611500.15776878660529328Illumina Single End PCR Primer 1 (100% over 30bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG576310.1486896637914907No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGGA503990.1300308924958328Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG482800.12456381058550384Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA398230.10274450349930651Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG393180.10144158874483926Illumina Single End PCR Primer 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)